This article describes how to add a text annotation to a plot generated using ggplot2 package.
The functions below can be used :
Its also possible to use the R package ggrepel, which is an extension and provides geom for ggplot2 to repel overlapping text labels away from each other.
Well start by describing how to use ggplot2 official functions for adding text annotations. In the last sections, examples using ggrepel extensions are provided.
# Install ggplot2
install.packages("ggplot2")
# Install ggrepel
install.packages("ggrepel")
Well use a subset of mtcars data. The function sample() can be used to randomly extract 10 rows:
# Subset 10 rows
set.seed(1234)
ss <- sample(1:32, 10)
df <- mtcars[ss, ]
library(ggplot2)
# Simple scatter plot
sp <- ggplot(df, aes(wt, mpg, label = rownames(df)))+
geom_point()
# Add texts
sp + geom_text()
# Change the size of the texts
sp + geom_text(size=6)
# Change vertical and horizontal adjustement
sp + geom_text(hjust=0, vjust=0)
# Change fontface. Allowed values : 1(normal),
# 2(bold), 3(italic), 4(bold.italic)
sp + geom_text(aes(fontface=2))
sp + geom_text(family = "Times New Roman")
sp + geom_label()
Others useful arguments for geom_text() and geom_label() are:
Its possible to change the appearance of the texts using aesthetics (color, size, ) :
sp2 <- ggplot(mtcars, aes(x=wt, y=mpg, label=rownames(mtcars)))+
geom_point()
# Color by groups
sp2 + geom_text(aes(color=factor(cyl)))
# Set the size of the text using a continuous variable
sp2 + geom_text(aes(size=wt))
# Define size range
sp2 + geom_text(aes(size=wt)) + scale_size(range=c(3,6))
The functions geom_text() and annotate() can be used :
# Solution 1
sp2 + geom_text(x=3, y=30, label="Scatter plot")
# Solution 2
sp2 + annotate(geom="text", x=3, y=30, label="Scatter plot",
color="red")
The functions annotation_custom() and textGrob() are used to add static annotations which are the same in every panel.The grid package is required :
library(grid)
# Create a text
grob <- grobTree(textGrob("Scatter plot", x=0.1, y=0.95, hjust=0,
gp=gpar(col="red", fontsize=13, fontface="italic")))
# Plot
sp2 + annotation_custom(grob)
Facet : In the plot below, the annotation is at the same place (in each facet) even if the axis scales vary.
sp2 + annotation_custom(grob)+facet_wrap(~cyl, scales="free")
There are two important functions in ggrepel R packages:
We start by creating a simple scatter plot using a subset of the mtcars data set containing 15 rows.
# Take a subset of 15 random points
set.seed(1234)
ss <- sample(1:32, 15)
df <- mtcars[ss, ]
p <- ggplot(df, aes(wt, mpg)) +
geom_point(color = 'red') +
theme_classic(base_size = 10)
# Add text annotations using ggplot2::geom_text
p + geom_text(aes(label = rownames(df)),
size = 3.5)
# Use ggrepel::geom_text_repel
require("ggrepel")
set.seed(42)
p + geom_text_repel(aes(label = rownames(df)),
size = 3.5)
# Use ggrepel::geom_label_repel and
# Change color by groups
set.seed(42)
p + geom_label_repel(aes(label = rownames(df),
fill = factor(cyl)), color = 'white',
size = 3.5) +
theme(legend.position = "bottom")
genes <- read.table("https://gist.githubusercontent.com/stephenturner/806e31fce55a8b7175af/raw/1a507c4c3f9f1baaa3a69187223ff3d3050628d4/results.txt", header = TRUE)
genes$Significant <- ifelse(genes$padj < 0.05, "FDR < 0.05", "Not Sig")
ggplot(genes, aes(x = log2FoldChange, y = -log10(pvalue))) +
geom_point(aes(color = Significant)) +
scale_color_manual(values = c("red", "grey")) +
theme_bw(base_size = 12) + theme(legend.position = "bottom") +
geom_text_repel(
data = subset(genes, padj < 0.05),
aes(label = Gene),
size = 5,
box.padding = unit(0.35, "lines"),
point.padding = unit(0.3, "lines")
)